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4 Results

4.1.3 Bacteriostatic effect on S. aureus is mediated by S. aureus speciﬁc IgGs

To ensure that the observed inhibitory effect mediated by IVIG is not due to changes in cul-

turing conditions by the addition of protein, the experiments were performed using 13 mg / ml

BSA or IVIG adjusted in PBS, respectively. This concentration of IVIG equates to 25 %

IVIG as used in previously described experiments. In contrast to IVIG treatment the co-

incubation of S. aureus with BSA led to signiﬁcant but only minor inhibition of growth (Fig.

4.3 A), thereby indicating that bacteriostasis is a speciﬁc effect mediated by IVIG.

control LB BSA IVIG

100

125

***

120 min

CFU / ml

(% of control)

control LB IVIG-DS IVIG

100

125

***

120 min

CFU / ml

(% of control)

Figure 4.3:

Effect of BSA or IVIG-DS on in vitro growth of S. aureus. Growth kinetics of S. aureus in LB

and upon addition of 13 mg / ml BSA (A) or dialysed IVIG (IVIG-DS; B) compared to IVIG in PBS

was assessed using a CFU enumeration assay. Samples supplemented with 50 % PBS served as

control. Shown are mean and SD of triplicates in percent of PBS control 120 minutes after addition

of the respective substances. Statistical signiﬁcance between indicated samples (capped lines)

was determined by one-way ANOVA followed by Bonferroni post analysis. P-values are indicated

by asterisks (∗ ∗ ∗ = p < 0.001).

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