Crosley CRSH268MW2 Bedienungsanleitung PDF herunterladen (Seite 47)

4 Results

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2155

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hp2160

2158

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10pI

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kDa

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Figure 4.10:

Subtractive proteome analysis (SUPRA) of S. aureus ACW proteins using IVIG and dSaIVIG

ACW proteins isolated from S. aureus strain ATCC 29213 were separated according to their iso-

electric points on pI 3 to 10 IPG strips. Gels loaded with 100 µg protein were used for subtractive

immunoblotting. Spots not immunoreactive with dSaIVIG (A) but immunoreactive with IVIG (B)

were spots predicted to be promising vaccine candidates (circles). Spots immunoreactive with

both IVIG and dSaIVIG were excluded from further investigation. For MALDI-TOF identiﬁcation

corresponding spots were matched with preparative gels loaded with 500 µg protein (C and D).

SUPRA was performed in collaboration with Eva Glowalla.

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