Crosley CRSH268MW2 Bedienungsanleitung PDF herunterladen (Seite 53)

4 Results

challenged intravenously with 3 x 10

CFU of S. aureus strain ATCC 29213 one week after

the second booster immunisation. For two weeks infected mice were monitored daily for

clinical signs of infection and mortality. Mice immunised with BSA as non-speciﬁc antigen

served as control.

As shown in Figure 4.14, 60 % of the mice immunised with either BT1 (A) or BT3 (C)

survived, in contrast to only 25 % surviving mice in the group of BSA-immunised mice. In

case of immunisation with recombinant BT2 (B) no improved survival compared to infected

control mice was observed.

To ascertain, whether immunisation with both candidates could potentiate the observed

protective effect, mice were immunised with a mixture of equal amounts of each antigen,

resulting in a serum titre comparable to single immunisation or BSA as control. As shown in

Figure 4.15, more than 65 % of the mice immunised with BT1 alone (A) survived the chal-

lenge with S. aureus in contrast to only 10 % in the BSA control group. In mice immunised

with BT3 (B) still 30 % more animals survived the challenge compared to BSA immunised

mice. In case of immunisation with a mix of recombinant BT1 and BT3 (C) the survival

rate further declined to 30 %, thus just 20 % higher than observed for mock immunised

mice. The discrepancy between single target immunisation and the bivalent immunisation

could be due to the lower amount of antigen used for immunisation, since only half of the

amount of each antigen was used for immunisation. Thus, the conditions for polyvalent

immunisations have to be further improved.

Altogether, three of the so far characterised proteins, namely hp2160, BT1 and BT3,

conferred protection in a murine model of sepsis. The remaining candidates will now be

tested consecutively, to enable the formulation of a multivalent mix of vaccine candidates

for active immunisation in order to improve the protective effects observed upon monovalent

immunisation.

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Crosley CRSH268MW2 Bedienungsanleitung Seite 53