Crosley CRSH268MW2 Bedienungsanleitung PDF herunterladen (Seite 15)

3 Material and Methods

3.1 Material

3.1.1 Chemicals and enzymes

Chemicals were of research grade and purchased from AppliChem, Merck, Sigma-Aldrich,

ROTH or Becton Dickinson GmbH unless stated otherwise. Buffers and solutions were

obtained from Bio-Rad or prepared using bi-distilled H

O from an EASYpure UV/UF H

puriﬁcation unit (Werner Reinstwassersysteme), degassed and sterilised by autoclaving or

ﬁltration through a 0.2 µm membrane, if necessary. Restriction enzymes were obtained

from Fermentas and used in recommended buffers. PFU Polymerase was obtained from

Promega and recombinant Lysostaphin from Sigma-Aldrich.

3.1.2 Bacteria and culture media

E. Coli Genotype Provider

E. coli K12 XL1Blue

recA1 endA1 gyrA96 thi-1 hsdR17

StratagenesupE44 relA1 lac [F’ proAB lacI

Z∆M15

Tn10 (Tet,

)]

DH5α

F-φ80dlacZ∆M15∆(lacZYA-argF)U169

InvitrogenrecA1 endA1 tonA hsdR17(r

)

phoA supE44 λ -thi-1 gyrA96 relA1

BL 21 F- dcm ompT hsdS (r

) gal Stratagene

S. aureus Resistance Provider

ATCC 29213 MSSA American Type Culture Collection

ATCC 29213-GFP

Ampicillin/ Inst. for Med. Microbiology,

Chloramphenicol Immunology and Hygiene, S. Leggio

1 2 ... 10 11 12 13 14 15 16 17 18 19 20 ... 87 88

Keine Kommentare

Crosley CRSH268MW2 Bedienungsanleitung Seite 15