Crosley CRSH268MW2 Bedienungsanleitung Seite 44

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4 Results
microarray qPCR
t0 t30 t60
-4
-2
0
2
4
fold change
t0 t30 t60
-4
-2
0
2
4
fold change
A B
fur
catalase
t0 t30 t60
-4
-2
0
2
4
fold change
t0 t30 t60
-4
-2
0
2
4
fold change
C
D
fhuC fhuD2
t0 t30 t60
-15
-10
-5
0
5
10
15
fold change
t0 t30 t60
-30
-20
-10
0
10
20
30
fold change
E
F
ferritin ribA
Figure 4.8:
Comparison of qPCR results to microarray results. RNA of IVIG and dSaIVIG samples for
each time point (t0, t30 and t60) was reverse transcribed into cDNA in triplicate. The qPCR was
performed using primers specific for fur (A), catalase (B), fhuC (C), fhuD2 (D), ferritin (E) and
ribA (F). Shown is the fold change in expression of the respective gene in IVIG versus dSaIVIG
samples (white bars) compared to the respective microarray result (black bars) for each time point.
Represented is mean and SD. Red lines indicate a fold change of 1.5.
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